9:45 AM - *SB08.01.01
Bacterial Photoenzymes Entrapped in Polydopamine Thin Films for Photoconversion
Gianluca Farinola1,Danilo Vona1,Rossella Labarile1,Gabriella Buscemi1,2,Roberta Ragni1,Francesco Milano3,Massimo Trotta2
University degli Studi-Bari Aldo Moro1,Institute for Physical-Chemical Processes (IPCF), CNR2,CNR-ISPA, Institute of Sciences of Food Production3
Reaction Centers (RCs) are specific transmembrane proteins of photosynthetic microorganisms that convert photons into charge separated states, with extremely high photoconversion efficiency. Our research group has demonstrated that bioconjugation of tailored molecular organic antennas represents an efficient strategy to enhance the light harvesting capability of RC extracted from Rhodobacter sphaeroides R26 photosynthetic bacterium [1-3]. Moreover, RC based photoelectrochemical cells [4,5], photosensors  and photoactive transistors  have been developed. RCs have been also integrated in semiconducting thin films deposited onto metal electrodes , and stabilized in soft structures like vesicles and polymersomes  before integration in electronic systems. Recently, we have developed chemical methods to optimize the interface of Rhodobacter sphaeroides RCs with electrodes, avoiding protein denaturation. To this aim, an adhesive biocompatible polymer, polydopamine (PDA), has been used. PDA can be easily produced by oxidative polymerization of dopamine in mild aqueous aerated conditions. It acts as an efficient coating layer for RC photoenzymes, improving the electrode/protein interface without altering the enzyme photoactivity . PDA can be further functionalized to improve its light transmittance, allowing an increase of photocurrent generation of PDA coated RCs . Hence, this polymer can be envisaged as a promising biocompatible adhesive material to efficiently interface photoenzymes with electrodes in bioelectronic devices for solar energy conversion.
 F. Milano, R.R. Tangorra, O. Hassan Omar, R. Ragni, A. Operamolla, A. Agostiano, G.M. Farinola, M. Trotta, Angew. Chem. Int. Ed., 51(44), 11019-11023 (2012).
 O. Hassan Omar, S. La Gatta, R.R. Tangorra, F. Milano, R. Ragni, A. Operamolla, R. Argazzi, C. Chiorboli, A. Agostiano, M. Trotta, G. M. Farinola, Bioconjugate Chem. 27, 1614-1623 (2016).
 S. la Gatta, F. Milano, G. M. Farinola, A. Agostiano, M. Di Donato, A. Lapini, P. Foggi, M. Trotta, R. Ragni, Biochimica et Biophysica Acta-Bioenergetics, 1860 (4), 350-359 (2019).
 F. Milano, F. Ciriaco, M.Trotta, D. Chirizzi, V. De Leo, A. Agostiano, L.Valli, L. Giotta, M.R. Guascito, Electrochim. Acta, 293, 105-115 (2019).
 F. Milano, A. Punzi, R. Ragni, M. Trotta, G. M. Farinola, Adv. Funct. Mater., 29 (21), 1805521, (2019).
 M. Chatzipetrou, F. Milano, L. Giotta, D. Chirizzi, M. Trotta, M. Massaouti, M.R. Guascito, I. Zergioti, Electrochemistry Communications, 64, 46-50 (2016).
 M. Di Lauro, S. la Gatta, C.A. Bortolotti, V. Beni, V. Parkula, S. Drakopoulou, M. Giordani, M. Berto, F. Milano, T. Cramer, M. Murgia, A. Agostiano, G.M. Farinola, M. Trotta, F. Biscarini, Adv. Electronic Mater. 6 (1), 1900888 (2020).
 E. D. Glowacki, R. R. Tangorra, H. Coskun, D. Farka, A. Operamolla, Y. Kanbur, F. Milano, L. Giotta and N. S. Sariciftci, J. Mater. Chem. C, 3 (25), 6554-6564 (2015).
 R. R. Tangorra, A. Operamolla, F. Milano, O. Hassan Omar, J. Henrard, R. Comparelli, F. Italiano, A. Agostiano, V. De Leo, R. Marotta, A. Falqui, G.M. Farinola, Trotta M., Photochem Photobiol Sci., 14(10), 1844-1852 (2015).
 M. Lo Presti, M. M. Giangregorio, R. Ragni, L. Giotta, M. R. Guascito, R. Comparelli, E. Fanizza, R. R. Tangorra,
A. Agostiano, M. Losurdo, G. M. Farinola, F.Milano, M. Trotta Adv. Electron. Mater. 2000140, (2020).
 G. Buscemi, D. Vona, R. Ragni, R. Comparelli, M. Trotta, F. Milano, G. M. Farinola, Adv. Sustain. Syst., doi.org/10.1002/adsu.202000303 (2021).