John A. Carlisle Advanced Diamond Technologies, Inc.
Martin Eickhoff Technische Universitaet Muenchen
Jose A. Garrido Technische Universitaet Muenchen
Janos Voeroes University and ETH Zurich
Erika Johnston Genzyme Corporation
9:30 AM - **D1.1
Bionanoarrays Prepared by Massive Parallel Dip-Pen Nanolithography
Chad Mirkin 1 2 , Khalid Salaita 1 2 , Yuhuang Wang 1 2 , Rafael Vega 1 2 , Joseph Kakkassery 1 2 , Clifton Shen 1 2 , Daniel Maspoch 1 2 Show Abstract
1 International Institute for Nanotechnology, Northwestern University, Evanston, Illinois, United States, 2 Department of Chemistry, Northwestern University, Evanston, Illinois, United States
The emerging field of nanobiotechnology relies on precise patterning of biological molecules on surfaces with nanometer resolution. Examples include the generation of DNA, protein, virus, and cell arrays that have potential biosensing, proteomics, and theranostics applications. There are currently a number of techniques for generating nanoscale features of biological molecules. These include electron-beam lithography, Dip-Pen Nanolithography (DPN), nanografting, nanoimprint lithography, nanopipet deposition, and contact printing. Each of these techniques has a set of capabilities that differentiate it from the other ones, and they all possess strengths and weaknesses with regard to resolution, speed, materials compatibility, complexity, and cost.DPN, a scanning-probe-based lithography in which an AFM tip is used to generate nanoscale biological patterns by directly transferring biomolecules to a surface, offers a number of realized and potential advantages over other nanopatterning techniques. It is substrate general and the patterning is typically performed under ambient conditions, which is critical for biomolecules. However, in its current state of development, the biggest limitation of DPN is its speed, especially when carried out in the context of single pen experiments. In addition, the bionanoarrays generated by DPN thus far are limited to a surface area of 100 μm × 100 μm using conventional single pens. Recently, DPN has evolved from a serial-based single pen experiment to a parallel patterning tool for generating complex nanoscale features over a large area.In this talk, we will discuss the massive parallel writing capabilities of DPN using a 2-D tip-array consisting of 55,000 individual tips (NanoPrint Array™). We will also discuss the strategies that we employed to perfectly align all of the 55,000 tips with the substrate. High-throughput DPN allowed us to fabricate in 20 minutes 88 million 100 nm-size high-resolution features. The 2-D tip array was used in the direct and indirect write approaches to generate nanoscale biological features. In particular, we have generated fibronectin and biological lipids nanoarrays over 1 cm2 substrate areas.
10:00 AM - D1.2
Lab-on-a-Chip Devices with Nanoscale Surface Topography for Neural Electrophysiological Applications.
Ludovico Dell'Acqua-Bellavitis 1 3 , Richard Siegel 2 3 Show Abstract
1 Engineering Science, Rensselaer Polytechnic Institute, Troy, New York, United States, 3 Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, New York, United States, 2 Materials Science and Engineering, Rensselaer Polytechnic Institute, Troy, New York, United States
This study is aimed at developing and fabricating novel in vitro electrophysiological devices capable of concurrently enhancing cell-biomaterial interaction and signal discrimination and resolution. The approach combines (i) the design and fabrication of integrated circuit platform (IC) and (ii) the synthesis ex situ of electrically insulated and aligned conducting nanowire arrays within a single device for electrophysiological studies of neuronal cells. The IC platform, which features an array of electrically-insulated electrodes deposited on thermally-grown silicon dioxide, was fabricated at three different scales of resolution to enable recordings of field potentials, action potentials and ionic channel potentials, respectively, at the multicellular, intercellular and intracellular levels (1).A conducting gold-plated copper / anodized alumina composite construct was fabricated as the interface between neuronal cells and the IC platform. The fabrication process was comprised of the fol